Mexican Trypanosoma cruzi stocks: analysis of minicircle kDNA homologies by cross-hybridization
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منابع مشابه
Mexican Trypanosoma cruzi stocks: analysis of minicircle kDNA homologies by cross-hybridization.
Homologies of minicircle kDNA of 27 Mexican stocks were studied by cross-hybridization with four kDNA probes derived from three reference stocks belonging to groups Trypanosoma cruzi I (SO34 cl4 and Silvio) and T. cruzi II (MN) and one Mexican stock. High homologies were only observed with Silvio (six stocks) and Mexican probes (11 stocks). After 30 min exposure (low homology) additional stocks...
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Kinetoplast DNA (kDNA) was isolated from 56 stocks of Trypanosoma cruzi isolated from human patients, animals and insects from Brazil, Venezuela, Colombia and Costa Rica. Comparison of the patterns of digested kDNA on acrylamide gels led to the grouping of several stocks into two schizodemes. Schizodeme analysis was also performed using a set of 330-bp fragments representing all the variable re...
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We demonstrate the genetic transfer of DNA between eukaryotes from different kingdoms. The mitochondrial kinetoplast DNA (kDNA) of the intracellular parasite Trypanosoma cruzi is transferred to human patients with Chagas disease. This transfer was reproduced experimentally in rabbits and chickens. The kDNA is integrated into the host genome. In the human chromosomes, five loci were identified a...
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round amastigote form by binary fission in various tissues of the mammalian host. (reviewed in Teixeira [1987]). Research Laboratory American trypanosomiasis infections span an exten-Faculty of Medicine sive geographical area between 42ЊN in the United University of Brasília States to 43ЊS in Argentina. Human Chagas disease is Brasília considered the most significant parasitic disease in Brazil...
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The taxon Trypanosoma cruzi, causative agent of Chagas disease, is composed of several discrete typing units (DTUs) named TcI-TcVI, and Tcbat. The history of the taxon T. cruzi is known, even though several controversial aspects remain as the relationships between TcIII and TcIV. We analyzed cloned T. cruzi stocks pertaining to the seven DTUs by filter hybridization tests of PCR amplicons from ...
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ژورنال
عنوان ژورنال: Memórias do Instituto Oswaldo Cruz
سال: 2000
ISSN: 0074-0276
DOI: 10.1590/s0074-02762000000400005